RNADNA hybrid dot blot - Bio-protocol

slot blot apparatus
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Slot blotting 1 DNA preparation a Dilute DNA

nylon membranes using a slot blot apparatus The membranes were baked, hybridized to the AAV probe, washed, and autoradiographed at –70 C for 90 min The

slot sites no wagering Assemble the dot blot apparatus with a pre-wetted nylon membrane Load denatured viral DNA and linearized standard plasmid DNA solutions into the wells

slot machine code Soak the membrane with water for 10 min before placing it on a dot blot apparatus Cover unused wells on the membrane apparatus NOTE: Handle the membrane