OK to run ASUS motherboard with RAM in slots 2 and 4 instead of 1

ram slot 1 and 3 or 2 and 4
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1') to properly use dual-channel with only two sticks present Slots 2 & 4 would then be filled upon upgrade RAM Test Methodology Pt

Yes, the 2009 4,1 & 201012 5,1 Mac Pro single Xeons use RAM in adjacent pairs [unlike the old 2008 3,1 which looks like a knitting pattern

ram slot 3 and 4 not working so my task manager said 1 of 2 ram slots used, when i hover it saidslot 1: emptyslot 2: 4,0GB, 1600mhzbut it show 8gb of ram just finemy

ram slot 2 and 4 Motherboard Featuring Four RAM Slots · 1 Single RAM Stick · 2 Dual RAM Stick · 3 Triple RAM Stick · 4 Quad RAM Stick