PCIe x8 NIC in PCIe x16 slot

does it matter which pcie x16 slot i use
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Yes Lower PCIE x will always work in the higher x slots You can run a 1x card in 1x and up, the slot will just be larger than what the card

The smallest PCIe slot will run at x1 speed, while a full-size PCIe slot will run at x16 PCIe x16 slots are generally intended for use with Graphics Cards  Since no GPU can saturate PCIe x16 anyway, there is no real life difference Currently, true x16 only matters with storage applications

10 slot watch box This means that a PCIe x1 slot supports just one PCIe lane, whereas a PCIe x4 slot has four A PCIe x16 slot has 16 For PCIe PCIe versions can ABSOLUTELY affect your performance If you've bought a PCIe card and expect it to perform in a PCIe or PCIe slot